We are constantly working on novel bioimaging methods of amyloids - protein aggregates connected with neurodegenerative diseases like Alzheimer's or Parkinson's. One of our approaches is multiphoton microscopy, which we have already used to study the structure of amyloid superstructures - spherulites. We are also looking for new organic dyes that could be used for Near-Infrared Two-Photon Imaging.

Members affiliated with that project: Manuela Grelich-Mucha, Patryk Obstarczyk, Maciej Lipok, Agata Hajda, Anna Pniakowska

Publications:

• M. Grelich-Mucha, M. Lipok, M. Różycka, M. Samoć, J. Olesiak-Bańska, One- and Two-Photon Excited Autofluorescence of Lysozyme Amyloids, J. Phys. Chem. Lett. 2022, 13, 21, 4673–4681 link

• P. Obstarczyk, M. Lipok, A. Żak, P. Cwynar and J. Olesiak-Banska, Amyloid fibrils in superstructures – local ordering revealed by polarization analysis of two-photon excited autofluorescence Biomater. Sci., 2022,10, 1554-1561  link

• P. Obstarczyk, M. Lipok, M. Grelich-Mucha, M. Samoć and J. Olesiak-Bańska Two-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging J. Phys. Chem. Lett. 12, 1432–1437 (2021) link