Detection and imaging of amyloids is a vigorously investigated topic in various areas, from protein biochemistry, development of new photonic biomaterials to medical diagnostics of neurodegenerative diseases. Autofluorescence of amyloids characterized by emission maxima located between 340 and 450 nm is an intrinsic optical property of amyloid fibers for a variety of amyloidogenic proteins, however, little is known about the origin of this process. In our research we investigate the origin of amyloids autofluorescence and determine how factors such as amyloid sequence, secondary structure or morphology, influence this process.

Members affiliated with that project: Manuela Grelich-Mucha, Maciej Lipok, Patryk Obstarczyk

Check out our JoVE video about using polarization-sensitive two-photon microscopy for imaging bovine spherulites!

Publications:

• M. Grelich-Mucha, M. Lipok, M. Różycka, M. Samoć, J. Olesiak-Bańska, One- and Two-Photon Excited Autofluorescence of Lysozyme Amyloids, J. Phys. Chem. Lett. 2022, 13, 21, 4673–4681 link
• P. Obstarczyk, M. Lipok, A. Żak, P. Cwynar and J. Olesiak-Banska, Amyloid fibrils in superstructures – local ordering revealed by polarization analysis of two-photon excited autofluorescence Biomater. Sci., 2022,10, 1554-1561  link
• M. Grelich-Mucha, A. M. Garcia, V. Torbeev, K. Ożga, Ł. Berlicki, J. Olesiak-Banska Autofluorescence of Amyloids Determined by Enantiomeric Composition of Peptides J. Phys. Chem. B 125, 21, 5502–5510 (2021) link
• P. Obstarczyk, M. Lipok, M. Grelich-Mucha, M. Samoć and J. Olesiak-Bańska Two-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging J. Phys. Chem. Lett. 12, 1432–1437 (2021) link